ynbA [2019-07-02 09:40:53]

GTP-binding protein, ribosome-splitting factor, rescues translationally arrested ribosomes

Locus: BSU17430

Isoelectric point: 9.24

Molecular weight: 37.51 kDa

Protein length: 420 aa

Gene length: 1263 bp

Function: rescuing of translationally arrested ribosomes

Product: GTP-binding protein, ribosome-splitting factor

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Targets of second messengers → Targets of (p)ppGpp
• Groups of genes → GTP-binding proteins

Gene

Coordinates: 1,875,304 1,876,566

The protein

Catalyzed reaction/ biological activity

• binds and hydrolyzes GTP and readily exchanges GDP for GTP
• rescuing of translationally arrested ribosomes PubMed

Protein family

• GTP1/OBG family (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• YphC, YyaF, Era, YsxC, RbgA, ThdF, Obg, YqeH

Effectors of protein activity

• binds ppGpp PubMed, ppGpp may inhibit activity PubMed

Structure

• 5ADY (the protein from E. coli in complex with the ribosome, 41% identity) PubMed

Expression and Regulation

Operons

• Genes: ynbA-ynbB-glnR-glnA
  Description: PubMed

  Regulation

  • the mRNA is processed between glnR and glnA by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Mutant

• MGNA-A020 (ynbA::erm), available at the NBRP B. subtilis, Japan
• BKE17430 (ynbA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
• BKK17430 (ynbA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

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Original publications

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